Anti-STAT1 Purified Monoclonal Antibody

Product Name

Anti-STAT1 Purified Monoclonal Antibody

View all STAT1 Antibodies

SKU/Catalog Number

M00036-3

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-STAT1 Purified Monoclonal Antibody (Catalog# M00036-3). Tested in IP, WB, IHC-P application(s). This antibody reacts with Mouse, Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-STAT1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00036-3)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

SM1

Isotype

Mouse IgG2b

Immunogen

STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE). The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00036-3 is reactive to STAT1 in Human, Mouse

Applications

M00036-3 is guaranteed for IP, IHC-P, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

87.335kDa

Background of STAT1

STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections): 5 μg/ml; positive tissue: lung macrophages.
Western blotting: 1-2 μg/ml.

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-STAT1 Purified Monoclonal Antibody M00036-3

Detection of Human phosphorylated STAT1[p Ser727] and total STAT1 by Western Blot. Western blot shows lysate of human malignant melanoma cells (short-term culture derived from a patient) in response to interferon treatment to induce phosphorylation of STAT1 at Ser727. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Specific band was detected for phosphorylated STAT1[Ser727] by the antibody PSM1 (upper panel) and total STAT1 level by the antibody SM1 (lower panel).
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For STAT1 (Source: Uniprot.org, NCBI)

Alternative Names

CANDF7; DKFZp686B04100; ISGF-3; ISGF-3,91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription-1; STAT1; STAT91 STAT1 CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91 signal transducer and activator of transcription 1 signal transducer and activator of transcription 1-alpha/beta|signal transducer and activator of transcription 1, 91kD|signal transducer and activator of transcription 1, 91kDa|transcription factor ISGF-3 components p91/p84

For more info on STAT1, check out the STAT1 Infographic

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In this infographic, you will see the following information for STAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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